On-line Redox Derivatization Liquid Chromatography Using a Carbon Monolithic Column

Method development validation for corticoids in animal feed samples by liquid chromatography using a monolithic column.

A LC method for corticosteroids (CC) determination in poultry feed using a Chromolith column and UV detection has been developed and validated. The method development involved the optimization of different hydro-organic mobile phases using methanol or ACN as organic modifiers, flow rate, and temperature. The optimum separation was achieved at 40 degrees C using ACN/water (21:79 v/v) as mobile p...

Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography

In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained ...

Automated pre-column derivatization and its application to amino-acid analysis using high-performance liquid chromatography

Although chemical modification of analytes is a well-accepted adjunct to chromatography, little attention had been paid to the automation of pre-column derivatization techniques. An automated pre-column system would offer advantages when analyses are performed using labile derivatives; and also when the time between injections is long and so staff time spent on achieving maximum analytical outp...

Column Liquid Chromatography

Separation of gold nanoparticles with a monolithic silica capillary column in liquid chromatography.

A separation system for gold nanoparticles was developed using monolithic silica capillary columns with 50 µm i.d., which were prepared via in-situ sol-gel processes. Gold nanoparticles with five different average sizes were synthesized via reduction of tetrachloroauric acid (HAuCl(4)) under different synthesis conditions, and were evaluated by UV-visible spectrophotometry, dynamic light scatte...